Research

Our laboratory studies mechanisms of induction and repair of DNA in mammalian cells, and develops new microscopy methods . We use various imaging techniques combined with cell biology, biochemical, and molecular methods.  Ourstudies into fundamental mechanisms of maintaining genome stability are pertinent to understanding of basic mechanisms of life, and are relevant to medical diagnostics and designing new therapies.

Our research interests include (i) interactions between various chemical compounds (anticancer drugs, toxins and various molecules exhibiting affinity to DNA) and chromatin in live cells, (ii) internal architecture and protein dynamics in DNA repair foci, (iii) mechanisms of induction of DNA damage by visible light, DNA-intercalating drugs, and CRSPR/Cas9, (iv) the influence of hypoxia on repair of DNA damage, and (v) processes of saturation of DNA repair capacity

We study DNA damage induction and repair using live cell fluorescence imaging, FRAP, FLIM, FCS, BiFC, super-resolution imaging (dSTORM) and several methods optimized in our laboratory (induction of DNA single- or double-strand breaks by visible light (Solarczyk  et al. DNA Repair (Amst). 2012;11(12):996) or CRISPR/Cas9 (Wesołowska et al., in preparation)detection of individual double- or single-strand breaks by dSTRIDE and sSTRIDE methods (Kordon et al. Nucleic Acids Res. 2020;48(3):e14), and our dedicated software for quantitative analysis of DNA damage (Berniak et al., Cytometry A. 2013;83(10):913-24).

 

 

 

 

 

 

 

Contact

 

 

 

Cell Biophysics Department

Ul. Gronostajowa 7
30-387 Kraków
jerzy.dobrucki_at_uj.edu.pl
tel. +48 12 664 6382; fax. +48 12 664 5503
http://helios.wbbib.uj.edu.pl/